Sequences, Plasmids

Plasmid Sequences

wdt_ID Plasmid Sequences File Format Description or Information Plasmid Map Reference Reference PDF
1 pEC9 Plasmid cDNA encoding vEC9 CG
10889 b
Seq: Oct 2006
Includes: vector and T7 promoter
Hahn, H., Palmenberg, A.C., JVirology, 1995
2 pMwt Plasmid cDNA encoding vMwt CG
10961 b
Seq: Oct 2006
Includes: vector and T7 promoter
Martin, L.R., etal., J. Virology, 1996
3 Rz-pMwt Plasmid cDNA encoding Rz-vMwt CG
10296 b
Seq: Oct 2006
Includes: vector, T7 promoter and ribozyme seq
Fata-Hartley, C.L., Palmenberg, A.C., J. Virology, 2005
4 pMC0 Plasmid cDNA encoding vMC0 CG
10906 b
Seq: Oct 2006
Includes: vector and T7 promoter
Martin, L.R., etal., J. Virology, 1996
5 pC15 Plasmid cDNA encoding HRV C15 CG
10345 b
Seq: Dec 2010
Includes: Includes: vector and T7 promoter.Seq in fasta format.
Bochkov, Y.A., etal., Nature Med., 2011
6 pF/R-wt, 2006 Plasmid pF/R-wt
8665 b
Includes: CMV promoter, T7 promoter, Fluc gene, EMCV IRES (wt), Rluc gene, 3’ UTR, polyA site, and complete vector (derivative of CloneTech pIRES). This seq is the Oct-'06 version of the plasmid
Bochkov, Y.A., Palmenberg, A.C., BioTech, 2006
7 pF/R-wt, updated Plasmid pF/R-wt, updated
8665 b
Seq: Jan 2013
Includes: CMV promoter, T7 promoter, Fluc gene, EMCV IRES (wt), Rluc gene, 3’ UTR, polyA site, and complete vector (derivative of CloneTech pIRES). This seq and map were updated from previous Oct-'06 version of plasmid
Bochkov, Y.A., Palmenberg, A.C., BioTech, 2006
8 pF/L Plasmid pF/L
7925 b
Seq: Oct 2006
Includes: CMV promoter, T7 promoter, Fluc gene, EMCV IRES (wt), EMCV Leader gene, 3' UTR, polyA site, and complete vector (derivative of CloneTech pIRES)
Porter, F.W., etal., PNAS, 2006
12 pCDHR3 Plasmid pCDHR3-flag-Tyr529
8003 b
Seq: Sept 2016
Includes: transfection plasmid for eucaryotic flag-tagged CDHR3 expression. Cys529Tyr codon: 2246-2248 (UGY/UAY)
13 pHalo3xNLS plasmid pHalo3XNLS
4936 b
Seq: Feb 2009
Includes: Halotag gene linked to 3xNLS under control of CMV promoter. Will express protein after eucaryotic cell transfection
Porter, F.W., Palmenberg, A.C., J. Virology, 2009

Virus Sequences

wdt_ID Virus Sequences File Format Description or Information GenBank Referent
1 vE-R EMCV Rueckert strain CG
7835 b (no 3' polyA included)
Seq update: May 2006
polyC: C125UCUC3UC10
GB: NC_001479 (also: M81861)
2 vEC9 EMCV C9 recomb strain CG
7722 b (including 3' polyA of 10 b)
Seq: Dec 2005
polyC: C9
GB: D0288856
3 vM-M Mengo M strain CG
7761 b (no 3' polyA included)
Seq: Dec 2005 (rev)
polyC: C44UC10
GB: L22089
4 vMC0 Mengo M derivative recomb strain CG
7740 b (including 3' polyA of 20 b)
Seq: Dec 2005 (rev)
polyC: none
5 vMwt same as Rz-vMwt
6 Rz-vMwt Mengo M derivative recomb strain CG
7765 b (including 3' polyA of 10 b)
Seq: Dec 2005 (rev)
polyC: C44UC10
GB: D0294633
7 vC15a Rhino C15a recomb strain CG
7111 b (no 3' polyA included)
GB: GU219984